Laboratory Protocols

Field Methods

• Soil Pits
• Bulk Density
• Vegetation
• Hydrolab Instructions

Soil Processing

• Full Protocol - Soil Processing
• Soil Processing Procedures
• Grinding for EA/IRMS
• Soil Splitting

Soil Physical Characteristics

• Soil Texture
• Water Holding Capacity
• Soil Moisture Retention
• Bulk Density

Soil Biogeochemistry

• Loss on ignition (LOI)
• Inorganic Carbon removal (for EA/IRMS)
• Sample prep for EA/IRMS(post - IC removal)
• Soil pH and EC
• Inorganic carbon analysis
• ​Soil Nutrient Extractions

Water Sampling

• Bottle washing
• Sample bottle choice
• Filtering and POC determination

Soil Biology

• Acetylene Reduction Assay
• Chlorophyll-a Extraction
• DNA Extraction using the MO BIO PowerSoil DNA Kit​